Object Details
- Description (Brief)
- This silver metal canister once contained Sau 3AI, an enzyme commonly used in molecular biology. Sau 3AI belongs to a class of enzymes known as restriction enzymes, which are useful for their ability to cleave DNA only at locations containing specific sequences of nucleotides, the small chemical units which make up the longer DNA molecule. Sau 3AI recognizes the sequence GATC and will cut before the G.
- This particular canister of Sau 3AI was used to create recombinant DNA molecules at Genentech, a biotechnology company, in the early 1980s.
- Source:
- GeneON, “Sau3AI.” http://www.taq-dna.com/sau3ai-_91.html
- Location
- Currently not on view
- Data Source
- National Museum of American History
- user
- Genentech, Inc.
- date made
- 1985-09
- Credit Line
- Gift of Genentech
- Physical Description
- Solution in Tris-HCl, 20 mmol/l (overall active ingredients)
- NaCl, 50 mmol/l (overall active ingredients)
- EDTA, 0.1 mmol/l (overall active ingredients)
- Beta-mercaptoethanol, 5 mmol/l (overall active ingredients)
- glycerol, 50% (v/v) (overall active ingredients)
- metal (overall material)
- Sau 3AI (overall active ingredients)
- Measurements
- overall: 2 7/8 in x 1 1/4 in; 7.3025 cm x 3.175 cm
- Object Name
- Sau 3AI
